Optimization of Calcium Alginate Hydrogel Bioencapsulation of Acinetobacter junii B6, a Lipopeptide Biosurfactant Producer

Background: Biosurfactants are derived from microbes, plants, and animals. Acinetobacter junii B6 is a lipopeptide biosurfactant producer previously investigated for its structure, physicochemical, product aggregation properties. Objectives: In this study, we optimized the bioencapsulation of A. in calcium alginate hydrogel. Methods: was encapsulated using The formulation hydrogel full factorial approach. Sodium concentration, chloride hardening time were selected as main factors, surface tension response measure. A scanning electron microscope (SEM) used to study bead's morphology. Results: Scanning image showed rounded smooth beads. most production reduced (35.98 mN/m) observed at concentrations 1% chloride, (1%) sodium alginate, 15 minutes time. can be hydrogels producing optimum experimental design. Conclusions: This represents practical method optimizing produce biosurfactants.